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KMID : 0368419990420030194
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Journal of Plant Biology
1999 Volume.42 No. 3 p.194 ~ p.198
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Analysis of the Tissue-Specific S RNase Cene Promoter Associated with Self-Incompatibility in Tomato(Lycopersicon peruvianum L.)
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Kim, Myung Hee
Shin, Dong Ill/Park, Hee Sung/Kim, Dal Ung/Chung, Il Kyung
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Abstract
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To understand the expression pattern of the S RNase gene in the floral tissues associated with self-incompatibility (SI), promoter region of S_11 RNase gene was serially deleted and fused GUS. Five chimeric constructs containing a deleted promoter region of the S_11 RNase gene were constructed, and introduced into Nicotiana tabacum using Agrobacterium-mediated transformation. Northern blot analysis revealed that the GUS gene was expressed in the style, anther, and developing pollen of all stages in each transgenic tobacco plant The developing pollen expressed the same amount of GUS mRNA in all stages in transgenic tobacco plants. In addition, histochemical analysis showed GUS gene expression in vascular bundle, endothecium, stomium, and tapetum cells during pollen development in transgenic plants. From these results, it is speculated that SI of Lycopersicon peruvianum may occur through the interaction of S RNase expressed in both style and pollen tissues.
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